Breeding of transgenic plants may occasionally produce antibiotic-resistant bacteria, and a new experimental procedure may eliminate this danger. The September issue of Natural Biotechnology describes the technology that can accelerate the growth of transgenic tobacco and lettuce. In order to develop plants that are more salt and insect resistant, scientists must first introduce foreign genes into their embryos. But not all cells will accept foreign genes. The easiest way to screen for plant cells that contain the introduced gene is to kill cells that do not receive the foreign gene. Biologists usually pair the desired gene or transgene with another gene that makes the cell resistant to antibiotics, and then place all cells in a solution of the antibiotic to kill cells that are not resistant to the antibiotic. Michael Syvanen, a microbiologist at the University of California, said that this method is so effective in many plants that "all of the previous technologies used antibiotic-resistant genes."
However, there is a danger in this method: Experiments show that some of the transgenes can be transferred from the grown plants to the bacteria. This raises the concern that bacteria in nature may also be resistant to antibiotic genes. Syvanen said that no one has observed this phenomenon in nature yet, but it is theoretically possible. "This is only a matter of time." Now, the research team led by microbiologist Tim Kunkel of Rockefeller University. It has been demonstrated that at least two crops, lettuce and tobacco, can be genetically modified without the use of antibiotic resistance genes. Kunkel's team used the transgene paired with a isopentenyltransferase, which accelerates shoot formation in seedlings. Then, the research group immersed the plant seedlings in the steroids that accelerate the growth of the shoots so that the transgenic seedlings can be screened out and the remaining seedlings can be discarded. Kunkel says how effective this method is in other plants remains to be tested.

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