First, choose a good venue: Pleurotus ostreatus can be cultivated indoors, but also in outdoor cultivation. Indoor cultivation, can use empty houses; outdoor cultivation, can build plastic greenhouses, sheds and other facilities; at the same time can also be suitable for the transformation of vegetables, plastic greenhouses, ventilation holes, used for mushroom cultivation.

Second, the choice of ingredients Ingredients: The production of Pleurotus ostreatus with a culture material to use fresh, no mildew, no insects, do not contain pesticides or other harmful chemicals corn cob, bean straw, straw, wheat straw, peanut shell, before the ingredients should Place it in the sun for 2-3 days to kill the bacteria and pests in the feed.

Commonly used formulas are: 1 corncob 50%, bean straw 47%, bran 2%, lime 1%. 2 Sawdust 89%, bran 10%, lime 1%. 3 99% straw and 1% lime. 4 Wheat straw 85%, corn flour 15%. After preparing the culture materials, the materials should be thoroughly mixed, and add water and mix well. And stuffed more than 2 hours, so that the culture material to eat water. It is advisable that the culture material be subjected to heaping and fermenting treatment under conditions, so that not only its physical and chemical properties can be improved, but also the hazards of bacteria and diseases and insect pests can be reduced. The concrete method is: Spread a layer of 10 cm thick straw on the cement floor, pile the material into a round pile on the straw, and use a wooden stick to breathe the eye to the bottom of the material, and then cover the sack with a plastic bag or plastic film. When the central temperature of the stockpile rises to 55°C-60°C, it is maintained for 12-24 hours. When the temperature rises to 55°C-60°C twice, the temperature is maintained for 24 hours, and then it is piled once more. The material is discolored and scented, and fermentation can be completed. When the expected temperature dropped to 30°C, bagging was performed again.

Third, bagging inoculation: selection of high-density polyethylene film cylinder, width 20-22 cm, length 40-47 cm, thickness of 0.02-0.025 mm, dry material loaded 0.7-0.8 kg, using two bags mouth inoculation. The operation method is as follows: First, one end of the film tube is tied with a string, and from the other end, firstly, one layer of bacteria is loaded, and then the material is loaded into the proximity of the tube mouth, and a taper with a diameter of 2.5 cm is used to play from the top to the bottom. Ventilation holes, fill in the hole with a small truffle species, and then spread on the surface of a layer of bacteria capping, and then use polypropylene rope to tighten the bag mouth.

4. Pile up of bacteria: After the mushroom shed is disinfected, carry the seed bag from the species to the mushroom shed for heaping the bacteria. The stacking level is determined by the temperature level. When the temperature is high in the summer and autumn, 2-4 layers are stacked. The material bags are piled into a “#” shape, cross-discharged to facilitate heat dissipation and leave the access road; when the temperature is low in the spring and winter, the bacteria bed A layer of rice straw or wheat straw should be laid on top of 10-12 layers and covered with plastic film or straw curtains to increase the temperature of the pile and promote the growth of mycelium. The main points of germination management are: 1 Keep the temperature, insert thermometers between the bags regularly, pay attention to changes in stack temperature. The germination temperature is preferably 25°C to 30°C. When the temperature is higher than 30°C, the heap should be scattered in a timely manner to increase the ventilation volume to prevent the high temperature from burning out the hyphae. When the temperature is lower than 20°C, try to increase the temperature and keep warm. 2 Ventilation: The mushroom shed is ventilated 2-3 times a day for 30 minutes each time. When the temperature is high, it is ventilated sooner or later. When the temperature is low, it is ventilated at noon. 3 Keep dry. The relative humidity in the shed is 60%-70%. 4 The light should be dark, and the weak light is conducive to mycelial growth. Turn 5. After the stacking, it is turned every 5-7 days, and the lower material bag is smashed upwards. The upper layer is smashed downwards, and the inside and the outside are squatting so that the temperature is consistent and the bacteria are neat. 6 ventilation oxygen. The use of closed-end spurs with two ends, in the early stage of germination, the oxygen content in the bag can meet the needs of mycelial growth. With the increase of mycelial growth, the oxygen content in the bag is insufficient, which will affect the normal growth of mycelium. .

When 10-15 days after inoculation, the two mycelia of the bag are each 2-3 cm long, and they can be punctured 8-10 times at the back of the hyphal growth line with a pin around the bag. Or, use sharpened bamboo chopsticks to prick holes 3-4 from the mouth of the bag, or loosen the ropes that fasten the two ends of the bag a little. Use the natural tension of the bag mouth membrane after loosening the rope to allow fresh air to enter the bag. , To supplement oxygen by ventilation, to promote the healthy growth of mycelium.

V. Mushroom management: When the cultivation bag full mycelium into the mushroom shed 10 days or so can be formed buds, this time should be controlled at 13 °C -18 °C, relative humidity of 80% -90%, daily ventilation 3-5 Each time, 25-30 minutes, if the ventilation is poor, the mushroom body will be small and dense, and the ventilation time will affect the temperature and humidity. When the flat mushroom cap flattened and faded in time, it was harvested in time. After harvesting the first batch of mushrooms, the water is stopped for 2-3 days, and the remaining mushrooms in the shed and the remaining mushroom stalks are cleaned in a timely manner and water is sprayed into the bags. Control the temperature to maintain the humidity, promote the rapid recovery of mycelium, produce new mushroom buds, increase the number of mushrooms, and extend the time of mushroom harvesting. After 10-15 days, the second pick can be adopted, generally 3-4 won.

VTM Kit

VTM sampling kits are designed for the collection, preservation, and transport of viruses, chlamydia, mycoplasma, and ureaplasma urea. They are now widely used for sampling and transporting prior to virus detection. MandeLab's VTM kits provide a safe and convenient way to collect and transport virus samples.

Nasal swab kit virus sampling tube,Used to collect samples of secretions from the throat or nasal cavity, Disposable

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