Xilin bottle ultrasonic bottle washer operating procedures
PCR ( Polymerase Chain Reaction)
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Rt-Qpcr Instrument,Real-Time Fluorescence Quantitative Pcr Instrument,Real Time Fluorescence Quantitative Pcr System,Fluorescent Quantitative Thermal Cycler Pcr Nanjing Superyears Gene Technology Co., Ltd. , https://www.superyearsglobal.com Xilin bottle washing machine operating procedures
1. ready
(1) Check the equipment electrical switch, the instrument indicates, the equipment transmission is normal.
(2) Check if the dial bottle clutch is in the normal position, if it is in the neutral position, please adjust it.
(3) Close the machine power switch, turn on the speed switch, the inverter operation panel fluorescent screen displays the setting frequency. At this time, press the RUN key, the inverter works, and the motor speed gradually reaches the set value. Stop pressing the STOP button.
(4) Run the machine for 2 minutes to check whether the equipment and mechanical transmission structure are normal, whether the operation sound is normal, and the production can be performed normally.
2. Start the water inlet valve first, fill the liquid storage tank with water, open the salt-free valve, open the water injection valve, spray the water normally, open the water pump switch, fill the ultrasonic cleaning tank with water, adjust the water inlet, and make the tank Keep a certain water level (do not open the ultrasonic wave below the water level) and open the air valve. When the water level in the tank reaches the specified water level, the ultrasonic switch is turned on, and the ultrasonic cleaning machine enters the bottle washing state.
(1) The operator puts the vial into the bottle tray and turns on the speed switch to make the speed 10~26Hz (100~400/min 7ML). Press RUN to start production.
(2) When there is a bottle condition, first press the STOP button to stop, check the bottle bottle part, clean the broken bottle in time, adjust the bottle wheel, and then drive production.
(3) When the bottle is turned into the bottle wheel and the track in the bottle turntable, it is strictly forbidden to pour the bottle.
3. note
(1) Never open the ultrasonic wave below the water level.
(2) After the end of production, the ultrasonic wave must be turned off first, then the water should be discharged.
(3) If the ultrasonic wave does not work, please consult the professional maintenance personnel.
(4) To adjust the production amount, set the required operating frequency according to the inverter manual.
(5) It is strictly forbidden to pour the bottle into the track.
The staff should strictly abide by the operating procedures of the vial bottle washer to make the machine reach the good operating state.
It provides a way to make more copies of a portion of DNA.
We need to have PCR ingredients that include DNA portion, Buffer solution, Primer+DNA (tap polymerase), and Nucleotides during the preparation step.
In the step of PCR sequence, there are three steps in this section.
Step 1 Denaturation
The addition of heat is needed to separate the two strands of the DNA molecule.
Step 2 Annealing
Temperature, is going to be cool in this step, should allow the primers to bind to the specific segments.
Step 3 DNA Synthesis
To make more copies of the DNA.
Those three steps above keep repeating to make enough copies of the DNA fragments. It is the principle of how PCR machine works.