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Spodoptera litura F. (Spodoptera litura F.) is one of the most frequent gluttonous pests in crops in China, especially in the southern food zone, which seriously affects the yield and quality of vegetables. Spodoptera litura nuclear polyhedrosis virus (NPV) is one of the important methods to control its harm. According to studies, after the virus invades the insect body, the nutrients of the insect body are used to synthesize themselves, and in the process of virus proliferation, the enzyme system in the target insect defense system can be destroyed, and the activity of esterase and phenol oxidase can be changed to cause insect death. The use of insect virus insecticides to control vegetable pests can effectively reduce the amount of chemical pesticide residues in vegetables, and is one of the important means to achieve the production of pollution-free vegetables. However, the field application of a single virus insecticide is slow, the insecticidal spectrum is narrow, and it is often difficult for users to accept it. Therefore, it is difficult to popularize and apply a large area of ​​the virus insecticide. People try to mix the virus and other factors, such as virus and virus, virus and synergistic protein, virus and chemical substances, etc., to select synergistic factors, shorten the half-life time (LT50), and improve the field application effect. Using Spodoptera litura as the target insect, under the indoor conditions, the synergistic effects of two common chemical pesticides, chlorpyrifos (Losenber) and gallonitrile (dilution), and SLNPV were determined. Infection of the 4th instar larvae of Spodoptera litura with tested mixed virus suspensions can significantly increase the insecticidal effect, shorten the half-life time of larvae, and increase the final death of Spodoptera litura larvae when compared to the use of viruses and chemical pesticides alone. rate. This shows that SLNPV and two kinds of chemical pesticides have synergistic effects. In practical applications, viral insecticides are slow in the field. Through the screening of virus potentiators, suitable synergistic combinations are selected and applied to practical applications, which can effectively increase the insecticidal effect of virus in fields. In addition to increasing the insecticidal effect of the virus, it also reduces the amount of chemical pesticides used in the field, thereby reducing the chemical pesticide residues on the crop and the environmental pollution.